Objectives: Biofilms formed byPseudomonas aeruginosa(PA) andStaphylococcusaureus(SA) have been shown to be an important factor in the pathophysiology of chronic rhinosinusitis (CRS). As well, honey hasbeen used as an effective topical antimicrobialagent for years. Our objective is to determine the in vitro effect of honey against biofilms produced by PA and SA.
Study Design: In vitro testing of honey against bacterial biofilms.
Methods: We used apreviously established biofilm model to assess antibacterial activity of honey against 11 methicillin-susceptibleSA (MSSA), 11 methicillin-resistant SA (MRSA), and11 PA isolates. Honeys were tested against both planktonic and biofilm-grownbacteria.
Results: Honey was effective in killing 100 percent of the isolates in the plank tonic form.The bactericidal rates for the Sidr and Manuka honeys against MSSA, MRSA, and PA biofilmswere 63-82 percent, 73-63 percent, and 91-91 percent, respectively. Theserates were significantly higher (P< 0.001) than those seen with single antibiotics commonly used against SA.
Conclusion: Honey, whichis a natural, nontoxic, and inexpensive product, is effective in killing SA and PA bacterial biofilms. This intriguing observation may have important clinical implications and could lead to a new approach for treating refractory CRS.